ICSI is an acronym for IntraCytoplasmic Sperm Injection.
It is a procedure where a single sperm cell is manually injected directly into an unfertilized egg. ICSI is performed by highly skilled embryologist using very sensitive tools called micromanipulators to inject the sperm via tiny pipettes known as microinjectors. The procedure is performed with the assistance of an inverted microscope built into a specialized anti-vibration table.
Embryologists usually perform sperm selection by observing the sperm at high magnification and then choose sperm cells that have a strong forward progression to their movement (motility), as well as normal head shape and form (morphology). Occasionally and in the absence of motile, normal sperms, other forms of sperms including non-motile and immature sperms could be used as could be obtained during sperm surgical extraction techniques.